TOP 10 WAYS TO REMOVE BUBBLES IN A FLOW CELL

ACA flow analyzer version of the Top 10 List.

10.    Flick the waste line.

9.      Pinch and release the waste line.

8.      If there is no back pressure coil (A001309) try adding one.   

7.      Push "Max Cal" on the pump. 

6.      Use syringe to force DI water and surfactant mixture through flow cell. 

5.      Add debubbler.  

4.      Add some surfactant to the carrier or first reagent. 

3.      Degas reagents.  

2.      Yell at it !!!!

and the number 1 way to get rid of bubbles inside a flow cell...

1.      Rebuild flow cell with new quartz windows (A002927) and o-rings (A000689).